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分子别名（Synonym）
CD85J,LILRB1,CD85,ILT2,LIR1,MIR7
表达区间及表达系统（Source）
Human LILRB1, Fc Tag (CDJ-H5252) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - His 458 (Accession # D9IDM8-1).
Predicted N-terminus: Gly 24
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 73.8 kDa. The protein migrates as 90-105 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>92% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human LILRB1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 92% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human LILRB1, Fc Tag (Cat. No. CDJ-H5252) is more than 95% and the molecular weight of this protein is around 150-185 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human HLA-G & B2M Heterodimer Protein, His,Avitag (Cat. No. HLM-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human LILRB1, Fc Tag (Cat. No. CDJ-H5252) with a linear range of 2-39 ng/mL (Routinely tested).
Protocol
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