Human MANF Protein, His Tag (MALS verified)

分子别名（Synonym）

Mesencephalic astrocyte-derived neurotrophic factor,Arginine-Rich Protein,arginine-rich, mutated in early stage tumors,ARMET,ARP,MANF,mesencephalic astrocyte-derived neurotrophic facto,Protein ARMET

表达区间及表达系统（Source）

Human MANF Protein, His Tag (MAF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 182 (Accession # P55145).

Predicted N-terminus: Leu 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.0 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联（Conjugate）

Unconjugated

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

95% as determined by SDS-PAGE.

98% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程