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分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (MSN-HA2H9) is produced via conjugation of AF488 to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
Predicted N-terminus: Glu 296
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.8 kDa.
偶联（Conjugate）
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
活性（Bioactivity）-FACS
The activity of Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) was higher than other Competitor.
Protocol
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-Mesothelin CAR Expression
5e5 of anti-MSLN CAR-293 cells were stained with 100 μL of 3 μg/mL of AF488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). AF488 signal was used to evaluate the binding activity (QC tested).
FACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with AF488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
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