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Human MMP-3 Protein, His Tag (active enzyme, MALS verified)

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  • 分子别名（Synonym）

    CHDS6

  • 表达区间及表达系统（Source）

    Human MMP-3, His Tag (MM3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Thr 272 (Accession # P08254-1).

    Predicted N-terminus: Tyr 18

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    MMP-3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.7 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 3 months under sterile conditions.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    MMP-3 SDS-PAGE

    Human MMP-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    MMP-3 SEC-MALS

    The purity of Human MMP-3, His Tag (Cat. No. MM3-H52H3) is more than 85% and the molecular weight of this protein is around 30-43 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    MMP-3 ELISA

    Immobilized Human MMP-3, His Tag (Cat. No. MM3-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-MMP3 Polyclonal Antibody (Routinely tested).

    Protocol

  • 活性（Bioactivity）

    Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 . The specific activity is >422 pmol/min/µg (QC tested).

    Protocol

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背景介绍

Matrix metalloproteinase 3 (MMP-3), also known as stromelysin 1 and progelatinase, is encoded by the MMP3 gene. The expression of MMP3 is mainly regulated at the transcriptional level, where gene promoters respond to a variety of stimuli including growth factors, cytokines, carcinogens and proto-oncogene products. MMP-3 degrade II, III, IV, IX and X collagen, proteoglycan, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7 and MMP-9, so MMP-3 plays a key role in connective tissue remodeling. This enzyme is involved in wound repair, atherosclerotic progression, and tumor initiation. Studies of MMP-3 wild type and gene knockout in mice have shown that MMP-3 increases the permeability of the blood-brain barrier after traumatic injury. Nuclear MMP-3 works as a transcription factor and protease.

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药物研发进展
  • 英文全称：

    Matrix metalloproteinase 3

  • 中文全称：

    基质金属蛋白酶-3

  • 种类：

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  • 临床药物数量：

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  • 最高研发阶段：

    临床前

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