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分子别名（Synonym）
PDCD1,PD1,CD279,SLEB2
表达区间及表达系统（Source）
Biotinylated Human PD-1, Fc,Avitag,His Tag (PD1-H82F4) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Predicted N-terminus: Leu 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc tag and polyhistidine tag.
The protein has a calculated MW of 45.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition, and 115-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Biotinylated Human PD-1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) is more than 90% and the molecular weight of this protein is around 113-133 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) with a linear range of 10-156 ng/mL (QC tested).
Protocol
Immobilized Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) with a linear range of 10-40 ng/mL (Routinely tested).
Protocol
Immobilized Nivolumab at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258): Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2394 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat. No. PD1-H5229) with an affinity constant of 1.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) on SA Biosensor, can bind PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.42 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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