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分子别名（Synonym）
PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
表达区间及表达系统（Source）
Human PDGF-BB, premium grade (PDB-H4112) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).
Predicted N-terminus: Met
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-PDBH19 is the GMP version of this PDB-H4112. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stag to later clinical phases.Request for sequence蛋白结构（Molecular Characterization）
Human PDGF-BB, premium grade is a disulfide-linked homodimeric polypeptide chain containing 2×109 amino acids, and has a calculated MW of 12.4 kDa. The protein migrates as 13 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid - Sodium Citrate, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human PDGF-BB, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-CELL BASE
Human PDGF-BB, premium grade (Cat. No. PDB-H4112) stimulates proliferation of NIH/3T3 cells. The specific activity of Human PDGF-BB, premium grade is >5.00 x 10^5 IU/mg, which is calibrated against human PDGF-BB WHO International Standard (NIBSC code: 94/728) (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human PDGF-BB, premium grade (Cat. No. PDB-H4112) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. PDB-H5259) with a linear range of 1-39 ng/mL (QC tested).
Protocol
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