积分兑换
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分子别名（Synonym）
Postfusion glycoprotein F0/post-F protein (RSV)
表达区间及表达系统（Source）
HRSV (A) Fusion glycoprotein F0, His Tag (RSF-V52H6) is expressed from human 293 cells (HEK293).
Predicted N-terminus: Gln 26
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.2 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
After reconstitution, this product is stable after storage at -70°C for 3 months.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
- For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
HRSV (A) Post-fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is more than 90% and the molecular weight of this protein is around 165-185 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) and HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Recombinant Human Anti-RSV Antibody (AM14) with a linear range of 0.004-0.25 μg/mL. HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is verified not recoginized by Recombinant Human Anti-RSV Antibody (AM14) (Routinely tested).
Protocol
Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Recombinant Human Anti-RSV Antibody (D25) with a linear range of 0.1-2 ng/mL. HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is verified not recoginized by Recombinant Human Anti-RSV Antibody (D25) in low concentration (Routinely tested).
Protocol
Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Recombinant Mouse Anti-RSV F Antibody (5C4) with a linear range of 0.004-0.25 μg/mL. HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is verified not recoginized by Recombinant Mouse Anti-RSV F Antibody (5C4) (Routinely tested).
Protocol
Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) and HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Anti-RSV Fusion Protein Antibody (131-2A) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (3B4) with a linear range of 0.144-9.197 ng/mL (Routinely tested).
Protocol
Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (4G2) with a linear range of 0.049-1.563 ng/mL (Routinely tested).
Protocol
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