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SARS-CoV-2 Spike S2 Protein, His Tag

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  • 分子别名(Synonym)

    Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2

  • 表达区间及表达系统(Source)

    SARS-CoV-2 Spike S2, His Tag (S2N-C52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.

    Request for sequence

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Spike S2 SDS-PAGE

    SARS-CoV-2 Spike S2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性(Bioactivity)-ELISA

     Spike S2 ELISA

    Immobilized SARS-CoV-2 Spike S2, His Tag (Cat. No. S2N-C52H5) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.4-3 ng/mL (QC tested).

    Protocol

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背景介绍

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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