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Human STAT3 Protein, His Tag (MALS & SPR verified)

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  • 分子别名（Synonym）

    STAT3, signal transducer and activator of transcription 3

  • 表达区间及表达系统（Source）

    Human STAT3, His Tag (ST3-H5149) is expressed from E. coli cells. It contains AA Gly 127 - Arg 688 (Accession # P40763-1).

    Predicted N-terminus: Met

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    STAT3 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 66.4 kDa. The protein migrates as 66-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    STAT3 SDS-PAGE

    Human STAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    STAT3 SEC-MALS

    The purity of Human STAT3, His Tag (Cat. No. ST3-H5149) is more than 85% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-SPR

    STAT3 SPR

    Human STAT3, His Tag (Cat. No. ST3-H5149) immobilized on CM5 Chip can bind STAT3 degrader with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

  • 活性（Bioactivity）-BLI

    STAT3 BLI

    Loaded Human STAT3, His Tag (Cat. No. ST3-H5149) on NTA Biosensor, can bind STAT3 degrader with an affinity constant of 29.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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背景介绍

Signal transducer and activator of transcription 3 (STAT3) regulates the expression of genes important for essential cellular processes including survival, proliferation, self-renewal, angiogenesis, and immune response. As an early tumor diagnostic marker, it is considered that constitutive STAT3 activation is a sufficient factor to cause malignant transformation and tumor development in model systems. The classical IL-6/STAT3 signaling pathway in cancer cells. IL-6 binds to the membrane-bound IL-6 receptor α (IL-6R) and IL-6 receptor β (also known as gp130). The IL-6/IL-6R/gp130 complex activate the phosphorylation of JAKs, followed by STAT3 phosphorylation and activation. Then, phosphorylated STAT3 forms a homodimer and translocates into the nucleus to bind to the promotor region of target genes and activates target gene transcription.

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  • 英文全称：

    Signal transducer and activator of transcription 3

  • 中文全称：

    信号转导和转录激活因子-3

  • 种类：

  • 上市药物数量：

    3 详情

  • 临床药物数量：

    19 详情

  • 最高研发阶段：

    批准上市

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