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Human Thrombopoietin / TPO Protein, premium grade

COAMS
DS
  • GMP version GMP-THNH25 is now available for seamless transition.
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  • 分子别名（Synonym）

    THPO,MGC163194,MGDF,MKCSF,ML,MPLLG,TPO,THCYT1

  • 表达区间及表达系统（Source）

    Human Thrombopoietin Protein, premium grade (THN-H5216) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).

    Predicted N-terminus: Ser 22

    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
    GMP-THNH25 is the GMP version of this THN-H5216. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stag to later clinical phases.

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Thrombopoietin Structure

    This protein carries no "tag".

    The protein has a calculated MW of 35.5 kDa. The protein migrates as 75 kDa±5 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 0.01 EU per μg by the LAL method.

  • 宿主蛋白残留（Host Cell Protein）

    0.5 ng/µg of protein tested by ELISA.

  • 宿主核酸残留（Host Cell DNA）

    0.02 ng/μg of protein tested by qPCR.

  • 无菌（Sterility）

    Negative

  • 支原体（Mycoplasma）

    Negative.

  • 纯度（Purity）

    95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in 20 mM NaAc-HAc, pH5.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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  • 电泳（SDS-PAGE）

    Thrombopoietin SDS-PAGE

    Human Thrombopoietin Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • 活性（Bioactivity）-CELL BASE

    Thrombopoietin CELL

    Human Thrombopoietin Protein, premium grade (Cat. No. THN-H5216) stimulates proliferation of Mo7e cells. The specific activity of Human Thrombopoietin Protein, premium grade is > 1.00ⅹ10^7 IU/mg, which is calibrated against human TPO Standard (NIBSC code: 03/124) (QC tested).

    Protocol

  • 活性（Bioactivity）-SPR

    Thrombopoietin SPR

    Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) captured on CM5 chip via anti-His antibody can bind Human Thrombopoietin Protein, premium grade (Cat. No. THN-H5216) with an affinity constant of 2.96 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

  • 稳定性（Stability）

    Thrombopoietin STABILITY

    The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG Thrombopoietin.

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背景介绍

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10-13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Both full length Tpo and shorter forms circulate in the plasma, with the shorter, N‑terminal EPO-like domain forms showing significantly increased specific activity (4, 5, 15). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (16, 17).

前沿进展

 
药物研发进展
  • 英文全称：

    Thrombopoietin

  • 中文全称：

    血小板生成素

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    1 详情

  • 最高研发阶段：

    临床一期

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