积分兑换
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分子别名（Synonym）
TSLP
表达区间及表达系统（Source）
Human TSLP Protein (R127A, R130A), His Tag (TSP-H5243) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.6 kDa. The protein migrates as 20-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human TSLP Protein (R127A, R130A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) is more than 90% and the molecular weight of this protein is around 19-27 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) at 2 μg/mL (100 μL/well) can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with a linear range of 0.15-0.6 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) with an affinity constant of 23 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1 μg/mL of Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) and negative control protein respectively, washed and then followed by PE anti-His Tag Antibody and analyzed with FACS (Routinely tested).
Protocol
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