积分兑换
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分子别名（Synonym）
TSLP
表达区间及表达系统（Source）
Human TSLP (R127A, R130A) Protein, His Tag (TSP-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).
Predicted N-terminus: Tyr 29
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.7 kDa. The protein migrates as 25-29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human TSLP (R127A, R130A) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human TSLP (R127A, R130A) Protein, His Tag (Cat. No. TSP-H52Ha) is more than 90% and the molecular weight of this protein is around 18-26 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with a linear range of 3-50 ng/mL (QC tested).
Protocol
Immobilized Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) at 1 μg/mL (100 μL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Captured Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 4.22 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Monoclonal Anti-Human TSLP Antibody, Human IgG2 on AHC Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 0.153 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1 μg/mL of Human TSLP (R127A, R130A) Protein, His Tag (Cat. No. TSP-H52Ha) and negative control protein respectively, washed and then followed by PE anti-His Tag Antibody and analyzed with FACS (Routinely tested).
Protocol
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