Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET)

优势与特点（FAB）

1. Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
2. Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
3. Simple and fast operation - No complicated washing steps, significantly reducing time.
4. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
5. Accurate and reliable results - High sensitivity with minimal matrix effects.
6. High throughput capability - Supports 500 tests, ideal for high-throughput screening.
7. Fast completion - Results in just 1 hour.

产品参数（Product Specifications）

产品概述（Product Overview）

The Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to measure the interaction between human CD32a (H167) and antibody drug candidates or CD32a (H167) inhibitors. It is designed to facilitate the ADCC and ADCP functional performance evaluation of antibody drug candidates, high-throughput screening of CD32a (H167) inhibitors within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of antibody drugs to bind to human CD32a (H167).

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

组分（Materials Provided）

原理（Assay Principles）

Human Fc gamma RIIA / CD32a (H167) binding kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use the mixture of biotinylated human Fc gamma RIIA / CD32a (H167) and Europium-chelate labeled streptavidin as the donor, FA labeled Human IgG1 antibody as the acceptor.

- In the absence of human Fc gamma RIIA/CD32a (H167) binding components, the donor and acceptor are in close proximity due to the binding of human Fc gamma RIIA/CD32a (H167) and FA-labeled Human IgG1 antibody. Under specific light source excitation, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission.

- In the presence of human Fc gamma RIIA/CD32a (H167) binding components, they prevent the donor-acceptor interaction, thereby inhibiting FRET from occurring.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程