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产品概述（Product Details）
ClinMax™ Human Interferon-γ(IFN-γ) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IFN-gamma that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human IFN-gamma in serum, plasma and cell culture supernatants.
It is suitable for potency assay of CAR-T/NK cell therapy.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
Assay TypeSandwich-ELISAAnalyteIFN-γFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity5.309 pg/mLAssay Time2 hr 50 minSample volume100 μLRange19.5 pg/mL-1250 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code87/586Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCRS017-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plateCRS017-C02Human IFN-γ Standard30 μgCRS017-C03Biotin-Anti-IFN-γ Antibody25 μgCRS017-C04Streptavidin-HRP50 μLCRS017-C0510xWashing Buffer50 mLCRS017-C062xDilution Buffer50 mLCRS017-C07Substrate Solution12 mLCRS017-C08Stop Solution7 mL质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IFN-γ is less than 5.309 pg/mL.
样本值（Sample Values）
Cell Culture Supernatant – Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using anti-CD3&CD28 magnetic beads and several concentrations of lipopolysaccharide (LPS) then detected IFN-γ secretion amount on Day 3 (72 hours).
稀释线性（Dilution Linearity）
High concentrations of human IFN-γ were added to different diluted substrates for gradient dilution to evaluate the linearity of the assay.
批内差异（Intra-Assay Statistics）
20 replicates of each of 3 samples containing different IFN-γ concentrations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
3 samples containing different concentrations of IFN-γ were tested in independent assays. Acceptable criteria: CV<10%.
回收率（Recovery）
IFN-γ was spiked into 3 human serum samples, and then analyzed. The average recovery of IFN-γ for serum samples is 93.5%, range is 85.2% - 105.2%.
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