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优势与特点（FAB）
- Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
- Simple and fast operation - No complicated washing steps, significantly reducing time.
- High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
- Accurate and reliable results - High sensitivity with minimal matrix effects.
- High throughput capability - Supports 500 tests, ideal for high-throughput screening.
- Fast completion - Results in just 1 hour.
产品参数（Product Specifications）
Assay TypeInhibition-TR-FRETAnalyteAnti-IL-17A Neutralizing AntibodyFormat100T/500TReactivityHumanRegulatory StatusRUOSensitivityIC50=6.1nMStandard Curve Range0.13 nM-133.33 nMAssay Time1 hrSuitable Sample TypeFor Human IL-17A / IL-17RA Inhibitor ScreeningSample volume10 μL产品概述（Product Overview）
The Human IL-17A / IL-17RA Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screening for inhibitors of human IL-17A binding to human IL-17RA within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of human IL-17A to bind to human IL-17RA.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeFRTP011-C01Human IL-17A Protein Europium-chelate100 tests/500 testsFRTP011-C02FA Labeled Human IL-17RA Protein100 tests/500 testsFRTP011-C03Anti-IL-17A Neutralizing Antibody10 μg/100 tests 50 μg/500 testsDB-04TR-FRET Sample Dilution Buffer, pH7.450 mL/100 tests & 500 testsDB-05TR-FRET Detection Buffer, pH7.450 mL/100 tests & 500 tests原理（Assay Principles）
This Human IL-17A/IL-17RA Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use a mixture of biotinylated Human IL-17A and Europium-chelate labeled streptavidin as the Donor and FA labeled Human IL-17RA as the Acceptor.
— In the absence of inhibitors for human IL-17A binding to human IL-17RA, the Donor and Acceptor are in close proximity due to the binding of human IL-17A and human IL-17RA. Upon excitation with a specific light source, the Donor emits a 620 nm signal, which is absorbed by the Acceptor, resulting in a 665 nm emission.
— In the presence of inhibitors that block the binding of human IL-17A to human IL-17RA, the Donor-Acceptor interaction is disrupted, preventing FRET from occurring.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-TR-FRET
Please refer to DS document for the assay protocol.
Inhibition of Europium-chelate labeled Human IL-17A: FA labeled Human IL-17RA binding by Anti-IL-17A Neutralizing Antibody
Premix serial dilutions of Anti-IL-17A Neutralizing Antibody (1:2 serial dilution, from 20 μg/mL to 0. 02 μg/mL (133.33-0.13 nM)) and Human IL-17A Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human IL-17RA Protein and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 6.1 nM. The assay was performed according to the above-described Datasheet (QC tested).
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