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组分（Materials Provided）
IDComponentsSizeEP155-C01High-bind Plate1 plateEP155-C02Human Integrin α 4 β 725 μgEP155-C03Biotinylated Human MAdCAM-110 μgEP155-C04Anti-Integrin α 4 β 7 Neutralizing Antibody20 μgEP155-C05Streptavidin-HRP5 μgEP155-C06Coating Buffer12 mLEP155-C0710xWashing Buffer50 mLEP155-C08Blocking Buffer50 mLEP155-C09Substrate Solution12 mLEP155-C10Stop Solution7 mLEP155-C111mol/L CaCl₂1.5 mLEP155-C121mol/L MgCl₂1.5 mLEP155-C131mol/L MnCl₂1.5 mL产品概述（Product Overview）
The integrin α4β7 is heterodimeric cell surface receptors expressed on most leukocytes. Mucosal addressing cell adhesion molecule 1(MAdCAM-1) is an exclusive ligand for α4β7 integrin. The interaction of integrin α4β7 with addressin mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is central to IBD pathogenesis.
应用说明（Application）
This kit is developed for screening for inhibitors of human Integrin α 4 β 7 binding to human MAdCAM-1.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new Integrin α 4 β 7 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human MAdCAM-1 to immobilized human Integrin α 4 β 7 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated MAdCAM-1 protein, a human Integrin α 4 β 7 protein, an anti-Integrin α 4 β 7 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human Integrin α 4 β 7.
b) Add your molecule of interest to the tests.
c) Add human MAdCAM-1-Biotin to bind the coated Integrin α 4 β 7.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to Integrin α 4 β 7:MAdCAM-1 binding will be determined by comparing OD readings among different experimental groups.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF Integrin α 4 β 7: MAdCAM-1 [Biotinylated] BINDING BY ANTI-Integrin α 4 β 7 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-Integrin α 4 β 7 Neutralizing antibody (Catalog # EP155-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
INHIBITION OF Integrin α 4 β 7: MAdCAM-1 [Biotinylated] BINDING BY selective α4 integrin antagonist
Serial dilutions of selective α4 integrin antagonist (1:1 serial dilution, from 10 μg/mL to 0.1526μg/mL) was added into Integrin α 4 β 7: Biotinylated MAdCAM-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (Routinely tested).
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