选择语言

Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (MALS verified)

用户评价
货号-规格
价格
Qty.

HLG-H52E9-25ug
¥2940.00

HLG-H52E9-200ug
¥16800.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    HLA-G & B2M & Peptide (RIIPRHLQL)

  • 表达区间及表达系统(Source)

    Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-H52E9) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-G) & Ile 21 - Met 119 (B2M) & RIIPRHLQL peptide (Accession # P17693-1 (HLA-G) & P61769 (B2M) & RIIPRHLQL).

    Predicted N-terminus: Gly 25 & Arg

  • 蛋白结构(Molecular Characterization)

    Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is assembled by biotinylated monomer (HLM-H82E4) and streptavidin.

    Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Complex Protein is produced by co-expression of HLA and B2M loaded with RIIPRHLQL peptide. Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.3 kDa, 13.9 kDa and 13.3 kDa. The protein migrates as 42-45 kDa and 15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

推荐产品

数据展示

  • 电泳(SDS-PAGE)

    HLA-G & B2M & Peptide (RIIPRHLQL) SDS-PAGE

    Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    HLA-G & B2M & Peptide (RIIPRHLQL) SEC-MALS

    The purity of Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (Cat. No. HLG-H52E9) is more than 90% and the molecular weight of this protein is around 260-290 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     HLA-G & B2M & Peptide (RIIPRHLQL) ELISA

    Serial dilutions of Anti-LILRB2 Antibody, Human IgG1 were added into Human LILRB2, Fc Tag : Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (Cat. No. HLG-H52E9) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1486 μg/mL (QC tested).

    Protocol

用户评价
发表评论

背景介绍

Human leukocyte antigen-G (HLA-G) is a group of closely linked genes located on the short arm of human chromosome 6. Hla-g belongs to a non-classical major histocompatibility complex. MHC class I molecules are selectively highly expressed in extravvillous trophoblast cells invading the uterine decidual membrane.The gene structure of HLA-G is similar to that of HLA-A,HLA-B and HLA-C, but the termination code appears in advance so that the intracellular segment of protein product encoded by HLA-G is only 6 amino acids, which is significantly shorter than the 30 amino acids of classical HLA classI antigen. The Human HLA-G & B2M & RIIPRHLQL Complex Protein is a complex of HLA-G of the MHC Class I, B2M and RMFPNAPYL peptide.

前沿进展

 
联系我们
项目合作
查看更多项目
实验案例分析
查看更多案例分析
  • 产品基础信息
  • 产品详情
  • 推荐产品
  • 数据展示
  • 用户评价
  • 背景介绍