产品详情
分子别名(Synonym)
IL-15,Interleukin-15,MGC9721
表达区间及表达系统(Source)
Cynomolgus / Rhesus macaque IL-15, His Tag (IL5-C52H4) is expressed from human 293 cells (HEK293). It contains AA Asn 49 - Ser 162 (Accession # P48092-1).
Predicted N-terminus: Asn 49
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.9 kDa. The protein migrates as 14 kDa and 16-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Cynomolgus / Rhesus macaque IL-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA
Immobilized Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-15, His Tag (Cat. No. IL5-C52H4) with a linear range of 1-8 ng/mL (QC tested).
ProtocolImmobilized Anti-IL-15 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-15, His Tag (Cat. No. IL5-C52H4) with a linear range of 2-16 ng/mL (Routinely tested).
ProtocolImmobilized Cynomolgus / Rhesus macaque IL-15, His Tag (Cat. No. IL5-C52H4) at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (Cat. No. ILG-H5254) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
ProtocolImmobilized Cynomolgus / Rhesus macaque IL-15, His Tag (Cat. No. IL5-C52H4) at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Protocol
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