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分子别名（Synonym）
IL-18,Interleukin-18,IL18,IL-1 gamma,IGIF,IL1F4,IL-1F4
表达区间及表达系统（Source）
Human IL-18 Protein, premium grade (IL8-H5114) is expressed from E. coli cells. It contains AA Tyr 37 - Asp 193 (Accession # Q14116-1).
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-L18H16 is the GMP version of this IL8-H5114. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stag to later clinical phases.Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 18.2 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IL-18 Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-CELL BASE
Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) stimulates secretion of IFN-γ by KG-1 cells. The specific activity of Human IL-18 Protein, premium grade is > 3.00ⅹ10^6 IU/mg, which is calibrated against WHO Reference Reagent Interleukin-18 (Human rDNA derived) (NIBSC code: 03/200) (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) at 5 μg/mL (100 μL/well) can bind Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human IL-18 R1, Fc,Avitag (Cat. No. IL1-H82F9) captured on Protein A Chip can bind Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) with an affinity constant of 14.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) captured on Protein A Chip can bind Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) with an affinity constant of 0.781 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
稳定性（Stability）
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG IL-18.
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