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分子别名（Synonym）
IL2,TCGF,lymphokine,Interleukin 2
表达区间及表达系统（Source）
Human IL-2 Protein, Tag Free (IL2-H5215) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
Predicted N-terminus: Ala 21
Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 15.4 kDa. The protein migrates as 14 kDa and 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IL-2 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-2 Protein, Tag Free (Cat. No. IL2-H5215) is more than 95% and the molecular weight of this protein is around 14-22 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-CELL BASE
Human IL-2 Protein, Tag Free (Cat. No. IL2-H5215) stimulates proliferation of CTLL-2 cells. The specific activity of Human IL-2 Protein, Tag Free is > 0.60 x 10^7 IU/mg, which is calibrated against human IL-2 WHO International Standard (NIBSC code: 86/500) (QC tested).
Protocol
活性（Bioactivity）-Cell Proliferation Assay
Human IL-2 Protein, Tag Free (Cat. No. IL2-H5215) has higher bioactivity than imported competitors when activates T cell proliferation with CD3/CD28 Activation Magnetic Beads.
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