产品详情
分子别名(Synonym)
OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
表达区间及表达系统(Source)
Biotinylated Human OX40 Ligand, His,Avitag (OXL-H82Q5) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).
Predicted N-terminus: His
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 51.1 kDa. The protein migrates as 65-80 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Biotin
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Biotinylated Human OX40 Ligand, His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human OX40 Ligand, His,Avitag (Cat. No. OXL-H82Q5) is more than 90% and the molecular weight of this protein is around 57-78 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human OX40, Fc Tag (Cat. No. OX0-H5255) at 1 μg/mL (100 μL/well) can bind Biotinylated Human OX40 Ligand, His,Avitag (Cat. No. OXL-H82Q5) with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
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