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分子别名（Synonym）
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
表达区间及表达系统（Source）
Mouse SIRP alpha, Fc Tag (SIA-M5258) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # BAA13520.1).
Predicted N-terminus: Lys 32
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Mouse SIRP alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse SIRP alpha, Fc Tag (Cat. No. SIA-M5258) is more than 85% and the molecular weight of this protein is around 160-185 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) at 10 μg/mL (100 μL/well) can bind Mouse SIRP alpha, Fc Tag (Cat. No. SIA-M5258) with a linear range of 10-156 ng/ml (QC tested).
Protocol
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