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Fc Receptor Proteins

The efficacy of a therapeutic antibody not only depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors. The binding affinity of the Fc fragment towards FcRn (FCGRT&B2M) would predict the antibody's half-life, while that between the Fc fragment and FcγRIIIa (CD16a) would influence the antibody's ability to elicit ADCC (antibody dependent cell mediated cytotoxicity). Therefore, candidates must be tested against a panel of receptors during antibody engineering.
ACROBiosystems offers a comprehensive collection of Fc receptor products, including FcRn, FcγRs and their common variants, to expedite your antibody drug development.
New Product Recommendation:Human Fc gamma RI / CD64 Binding Kit（TR-FRET）, Human FcRn binding Kit (TR-FRET)
It is easy to use, highly sensitive, and accurate in results, thus supporting and accelerating the research and development process of antibody drugs.

Hot targets

Advantages of Fc receptor protein products

Expressed by HEK293 Cells: post-translational modification and proper protein folding

Multiple species:Human, Mouse, Cynomolgus/Rhesus macaque, Rat, Porcine, Rabbit, Feline, Bovine, can be fully applied to different cross species experiments

High purity:SDS-PAGE verification purity>95%, SEC-MALS verification purity>90%

Low endotoxin:<1.0 EU/µg

High stability:strict quality control to ensure high batch-to-batch consistency

Biotinylated Fc Receptor proteins labeled with Avitag™ offered:the labeling efficiency is high, and the labeling site is specific and clear, which is suitable for ELISA/SPR/BLI detection based on binding to streptavidin in the process of drug development and optimization process

Affinity verified by SPR & BLI: activity guaranteed, and protocols offered free of charge

Key features

High purity

To meet the high purity requirement of pharmaceutical applications, our production team perform both SDS-PAGE and SEC-MALS analyses to the Fc receptors for QC purposes. It's noteworthy that it's essential to use monomeric Fc receptor, which represents its natural state. The formation of oligomers during production may result in artificially enhanced binding interaction due to avidity effect. To avoid such situation, we have established strict internal control standards of monomer purity by SEC-MALS testing. Only those batches meeting all purity requirements are released.

Fc receptors-SPR
Fig. 1 The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 95% verified by SDS-PAGE. The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% verified by SEC-MALS. And the molecular weight of this protein is around 25-35 kDa.
Bioactivity validated by SPR&BLI

The binding affinity between two molecules can be determined by many methods. However, the studies of Fc interaction are often done by SPR, because traditional ELISA is not appropriate for low affinity receptors such as CD16 and CD32. To assure our Fc receptors deliver expected performance, our QC team use Biacore platform to test our products against reference antibodies. Additionally, to meet more applications in the development of antibody drugs, our QC team also use ForteBio platform to test our products against reference antibodies. All SPR&BLI protocols are available for free.

Fc receptors-SPR
Fig. 2 Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herc*ptin with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K).
Protocol
Fc receptors-BLI
Fig. 3 Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (Cat. No. FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herc*ptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).
Protocol
Fc receptors-BLI
Fig. 4 Loaded Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herc*ptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e).
Protocol
Fc receptors-BLI
Fig. 5 Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) on SA Biosensor via Biotin his antibody, can bind Herc*ptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e).
Protocol
High Batch-to-batch Consistency

We routinely apply rigorous quality control measures to ensure consistent performance of our product. As shown below, the batch variation among the tested samples is negligible.

Fc receptors-High batch to batch consistency
Fig. 6 Immobilized Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Ritux*mab with an affinity constant of 10 μM as determined in a SPR assay (Biacore T200).
Protocol

We have compared the performance of different batches of CD32b (CDB-H5228) in the same assay, and the software analysis showed that the similarity score was very high, meaning high batch-to-batch consistency.

Fc receptors-High batch to batch consistency
Fig. 7 Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The consistency of different batches is more than 90%.

Product list

FcRn
FcγRIII(CD16)
FcγRII(CD32)
FcγRI (CD64)
FcγRIV(CD16-2)
FcεR
FcαR1

Fc receptor related molecules

Latest citations

  • Introduction
  • Hot targets
  • Advantages of Fc receptor protein products
  • Key features
  • Product list
  • Fc receptor related molecules
  • Latest citations