关注公众号
收藏产品 产品详情
分子别名（Synonym）
IL-17A,Interleukin-17A,CTLA-8,IL-17
表达区间及表达系统（Source）
Human IL-17A Protein, His Tag (ILA-H5243) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (Accession # Q16552-1).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 17-20 kDa and 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IL-17A Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-17A Protein, His Tag (Cat. No. ILA-H5243) was greater than 90% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Human IL-17A Protein, His Tag (Cat. No. ILA-H5243) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL17A Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Human IL-17A Protein, His Tag (Cat. No. ILA-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, His,Avitag (Cat. No. ILR-H82E5) with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human IL-17 RA Protein, Fc Tag (Cat. No. ILA-H525f) captured on Protein A Chip can bind Human IL-17A Protein, His Tag (Cat. No. ILA-H5243) with an affinity constant of 0.360 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作