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SourceThis Monoclonal Anti-His Tag Antibody, Rabbit IgG is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Rabbit constant domain.SpecificityThis antibody specifically reacts with His tag proteins, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the his-tagged protein as Acceptor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (Cat. No. FRT-TA11) is used as the Acceptor. Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1), at 62.5 ng/well, can bind to the Human PD-L1, His Tag (Cat. No. PD1-H5229) with a linear detection range of 10-0.01 μg/mL (QC tested).
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