Product Details
Source
Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (4B8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
Clone
4B8
Species
Mouse
Isotype
Human IgG1 | Human Kappa
Conjugate
Unconjugated
Antibody Type
Recombinant Monoclonal
Reactivity
Virus
Immunogen
Recombinant Nipah virus Glycoprotein G is expressed from human 293 cells
Specificity
Specifically recognizes Nipah virus Glycoprotein G.
Application
ApplicationRecommended UsageWestern Blot10-0.5 μg/mLELISA0.06-31 ng/mLPurity
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (4B8) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (4B8) (Cat. No. GLN-MY2258) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Nipah virus Glycoprotein G, His Tag (Cat. No. GLN-N52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (4B8) (Cat. No. GLN-MY2258) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Western Blot

Detection of Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (4B8) by Western Blot. Monoclonal Anti-Nipah virus (NiV) Glycoprotein G Antibody, Human IgG1 (4B8) at 2 μg/mL dilution + Nipah virus Glycoprotein G, His Tag (MALS verified) at 800 ng.
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 73-90 kDa 12% Bis-Tris Protein Gel
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