Product Details
Features and Advantages
- Native Conformation: Native sequences, tag-free and natural function.
- Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
- Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
- Lowest Endotoxin level (<0.01 EU/ug).
- Safety Assurance: Sterile filtration through 0.2 μm membrane.
- AOF: Animal origin-free raw materials throughout the production process.
Source
Monoclonal Anti-Human CD28 Antibody, Research Grade (CD8-C112GN) is recombinantly produced from human 293 cells (HEK293).
Isotype
Mouse IgG1 | Mouse Kappa
Specificity
This product is a specific antibody specifically reacts with CD28.
Endotoxin
Less than 0.002 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
For long term storage, the product should be stored at liquid state at -70°C.
This product is stable after storage at:
- 2-8°C for 12 months under sterile conditions;
- -70°C for 24 months.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Performance Data
SDS-PAGE

Monoclonal Anti-Human CD28 Antibody, Research Grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Monoclonal Anti-Human CD28 Antibody, Research Grade (Cat. No. CD8-C112GN) is more than 95% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
Bioactivity-CELL BASE

Monoclonal Anti-Human CD28 Antibody, Research Grade (Cat. No. CD8-C112GN) stimulates secretion of IL-2 by PBMC stimulated with 4 ng/mL Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a). The typical EC50 for this effect is 0.8199 ng/mL (QC tested).
Protocol
Bioactivity-ELISA

Immobilized Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD28 Antibody, premium grade (Cat. No. CD8-C112GN) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews

Background
Recent Advances
- English Name:
T-cell-specific surface glycoprotein CD28
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
31 Details
- Highest Development Stage:
Phase 2 Clinical
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