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Biotinylated FMC63 scFv, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
CD9-BV4324b-25ug
$280.00
CD9-BV4324b-200ug
$1420.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Source

    Biotinylated FMC63 scFv, Fc,Avitag™ is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Species

    Mouse

  • Isotype

    C-Fc

  • Conjugate

    Biotin

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Human

  • Immunogen

    CD19

  • Specificity

    Specifically recognizes the CD19.

  • Application

    Application
    Recommended Usage
    ELISA
    10-20000 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FMC63 SDS-PAGE

    Biotinylated FMC63 scFv, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    FMC63 SEC-MALS

    The purity of Biotinylated FMC63 scFv, Fc,Avitag (Cat. No. CD9-BV4324b) is more than 95% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     FMC63 ELISA

    Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 1 μg/mL, add increasing concentrations of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) and then add Biotinylated FMC63 scFv, Fc,Avitag (Cat. No. CD9-BV4324b) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 78 ng/mL (QC tested).

    Protocol

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Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

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