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Alexa Fluor™ 647-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (M4H08) (Non-Neutralizing) (0.03% Proclin)

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Cat. No. / Size
Price
Qty
FM3-ABFMY2-25tests
$380.00
FM3-ABFMY2-200tests
$2280.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Source

    Monoclonal Anti-Mouse FMC63 antibody, Rabbit IgG1 (M4H08) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • Application

    Flow Cytometry.

  • Clone

    M4H08

  • Species

    Rabbit

  • Isotype

    Rabbit IgG | Rabbit Kappa

  • Specificity

    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

  • Reactivity

    Human

  • Immunogen

    Recombinant FMC63 scFv derived from HEK293 cells.

  • Conjugate

    AF647

    Excitation Wavelength: 640 nm

    Emission Wavelength: 672 nm

  • Isotype Control

    The isotype control is sold separately; please refer to Cat. No. DNP-AFM776 for product information.

  • Recommended Dilution

    1:20

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     FMC63 FACS

    Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with Alexa Fluor 647-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (M4H08) (Cat. No. FM3-ABFMY2) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. Alexa Fluor™ 647 signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

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