Save Product
Product Details
Source
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Clone
Y45
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Conjugate
Unconjugated
Antibody Type
Recombinant Monoclonal
Reactivity
Human
Immunogen
FMC63
Specificity
Specifically recognizes the antigen-recognition domain of FMC63 derived antibody.
Application
ApplicationRecommended UsageELISA6.25-50 ng/mLPurity
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 6 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) is more than 95% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) with a linear range of 0.1-6 ng/mL. FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) is verified not recoginized by Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) in low concentration (QC tested).
Protocol
ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) was inhibited by increasing concentration of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
