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resDetect™ Human Interferon-γ(IFN-γ) ELISA Kit (Residue Testing)

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Cat. No. / Size
Price
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RES-A028-96tests
$510.00
ETA of in-stock products:2 business days
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Product Details

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    IFN-γ
    Format
    96T (8×12 strips)
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    < 3.125 pg/mL
    Standard Curve Range
    3.125-200 pg/mL
    Assay Time
    2 hr 50 min
    Suitable Sample Type
    For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum.
    Sample volume
    100 μL

  • Product Overview

    Human Interferon-γ (IFN-γ) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IFN-γ residues in cell culture supernatants, serum, and plasma. The kit contains GMP Human Interferon-γ (IFN-γ) (ACROBiosystems, cat# GMP-IFGH24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human IFN-γ.

  • Reconstitution

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • Storage

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    RES028-C01
    Pre-coated Anti-IFN-γ Antibody Microplate
    1 plate (8×12 strips)
    RES028-C02
    Human IFN-γ Standard
    20 μg
    RES028-C03
    Biotin-Anti-IFN-γ Antibody
    50 μL
    RES028-C04
    Streptavidin-HRP
    50 μL
    RES028-C05
    10xWashing Buffer
    50 mL
    RES028-C06
    2xDilution Buffer
    50 mL
    RES028-C07
    Substrate Solution
    12 mL
    RES028-C08
    Stop Solution
    7 mL

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

     IFN-gamma TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation
  • Dilution Linearity

    To assess the linearity of the assay, samples spiked with high concentrations of human IFN-γ, were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

     IFN-gamma DILUTION LINEARITY
  • Intra-Assay Statistics

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV<10%.

     IFN-gamma INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.

     IFN-gamma INTER-ASSAY STATISTICS
  • Recovery

    Three parts of blank serum were added with different concentrations of human IFN-γ and the serum without human IFN-γ was used as background to calculate the recovery rate. The range of the recovery rate is 89.2-116.0%, and the average recovery is 98.3%.

     IFN-gamma RECOVERY

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Drug Development Progress
  • English Name:

    Interferon gamma

  • Category:

  • Approved Drugs:

    4 Details

  • Drugs in Clinical Trials:

    14 Details

  • Highest Development Stage:

    Approved

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