Save Product
Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteProtein LFormat96T (8×12 strips)Regulatory StatusRUOSensitivity＜50 pg/mLStandard Curve Range50 pg/mL-3200 pg/mLAssay Time2 hrSuitable Sample TypeFor the quantitative determination of recombinant protein LSample volume50 μLProduct Overview
Protein L ELISA Kit is based on the ELISA sandwich method and is used to measure natural or structurally conserved recombinant forms of Protein L, as well as recombinant forms of Protein L with significant structural differences from natural Protein A, such as MabSelect™ VL, in bioprocess manufacturing applications. It is designed to provide a reliable solution to aid in optimal purification process development and routine quality control of in-process streams and final products. It can also be used as a universal detection tool for the quantitative determination of Protein L.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES026-C01Pre-Coated Anti-Protein L Antibody Microplate1 plate (8×12 strips)RES026-C02Recombinant Protein L Standard (1 μg/mL)100 μLRES026-C03Biotin-Anti-Protein L Antibody150 μLRES026-C04Streptavidin-HRP10μgRES026-C0510×Sample Dilution Buffer15 mLRES026-C06Denaturation Buffer15 mLRES026-C0720×Washing Buffer30 mLRES026-C08Antibody Dilution Buffer15 mLRES026-C09Streptavidin-HRP Dilution Buffer15 mLRES026-C10Substrate Solution12 mLRES026-C11Stop Solution8 mLACRO Quality Management System
Customers Also Viewed
Performance Data
Typical Data
Please refer to DS document for the assay protocol.
Detection of Recombinant Protein L by sandwich-ELISA Assay. Immobilized Anti- Protein L Antibody can bind Recombinant Protein L . Detection was performed using Biotin-Anti- Protein L Antibody with sensitivity of 50 pg/mL. For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Intra-Assay Statistics
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Recovery
Add different concentrations of Protein L (0.2ng/mL、1ng/mL、10ng/mL) to different concentrations of Human IgG1 (Bevacizumab) (20mg/mL、10mg/mL、5mg/mL) or Human IgG4 (Toripalimab) (10mg/mL、5mg/mL、2mg/mL), then dilute the antibodies to a reasonable range, then test and calculated the concentration of protein L to give the recovery rate.
Interference effect
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
Specificity
Host cell protein (HCP 500 ng/mL) and host cell DNA (HCD 0.5 ng/mL) of HEK293, E.coli or CHO systems were added to human IgG1 (Bevacizumab, 1mg/mL) and human IgG4 (Toripalimab, 1mg/mL), respectively, which were higher than the usual quality standard limit. Then high, medium, and low concentrations of Protein L were added, respectively, and the ratio of Protein L recovery in the Protein L added samples without HCP and HCD was added as the specificity verification index.
Customer Reviews Writing Reviews
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
