Rat CD73 Protein, His Tag (active enzyme)

Synonym

CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

Source

Rat CD73, His Tag (CD3-R52H5) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 551 (Accession # P21588).

Predicted N-terminus: Trp 27

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 60.3 kDa. The protein migrates as 62-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20mM Tris,120mM NaCl,pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process