Product Details
Synonym
CTLA4, CD152
Source
Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (CT4-C82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88-1). In the region Ala 37 - Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus.
Predicted N-terminus: Ala 37
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 15.9 kDa. The protein migrates as 20-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (Cat. No. CT4-C82E5) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avitag,His Tag (Cat. No. CT4-C82E5) with a linear range of 2-63 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Cytotoxic T-Lymphocyte-Associated Antigen 4
- Category:
- Approved Drugs:
5 Details
- Drugs in Clinical Trials:
65 Details
- Highest Development Stage:
Approved
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