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Product Details
Features and Advantages
- Seamless Transition from Preclinical to Clinical: Same cell clone, process, and QC as GMP products.
- Enhanced Production Standards: AOF raw materials, pharma-grade excipients, Grade B+A (ISO 5) environment.
- Comprehensive Quality Control: Additional strict testing for process impurities, sterility, and mycoplasma.
- Cost-Effective Solution: GMP-comparable quality at an economical price for early development.
- Risk Mitigation: Enhanced safety through rigorous impurity control and comprehensive testing.
Synonym
Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
Source
Recombinant Fibronectin fragment, premium grade (FIN-H5116) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # P02751-15).
Predicted N-terminus: Met
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-FINH18 is the GMP version of this FIN-H5116. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stage to later clinical phases.Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Supplied as 0.2 μm filtered solution in 12.5 mM Citric acid, pH6.2 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C for 12 months under sterile conditions.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Performance Data
SDS-PAGE
Recombinant Fibronectin fragment, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) was greater than 90% as determined by SEC-HPLC.
Bioactivity-SPR
Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) immobilized on SA Chip can bind Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) with a typical affinity constant of 0.547 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) immobilized on SA Chip can bind Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) with a typical affinity constant of 38.2 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) immobilized on CM5 Chip can bind Heparin Sulfate with a typical affinity constant of 0.103 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Application Data
Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) could promote mesenchymal stem cell attachment and proliferation in several passages, comparable with Competitor T. The bioactivity of Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) is similar to GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18).
Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) could maintain the stemness of MSC in several passages, with high expression of MSC markers CD73, CD90 and CD105 and negative expression of HLA-DR, CD34 and CD45. The stemness ability of Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) is comparable with Competitor T and similar to GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18).
2e5 Jurkat cells were transfected with pLenti-CMV-EGFP-puro-Amp for 48 hours in the presence or absence of Fibronectin fragment, premium grade (Cat. No. FIN-H5116)-coated. The GFP fluorescence was detected with FACS. The Alexa Fluor 488 signal was used to evaluate the expression of GFP+ Jurkat cells (Routinely tested).
CD34+ CD45+ hematopoietic cells were seeded on GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coated plates and differentiated for 21 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD5 and CD7. GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) combined with GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) can induce the formation of CD7+ and CD5+ CD7+ T-cell progenitors. The bioactivity of GMP Recombinant Human Fibronectin fragment (Cat. No. GMP-FINH18) is similar to Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116), as well as the competitor T. Negative control: w/o Human Fibronectin.
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