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Product Details
Synonym
ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1
Source
Alexa Fluor 647-Labeled Human Her2, His Tag (HE2-HA2H5) is produced via conjugation of AF647 to Human Her2, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Her2, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
Predicted N-terminus: Thr 23
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Evaluation of CAR expression
FACS Analysis of Anti-Her2 CAR Expression
5e5 of anti-Her2 CAR-293 cells were stained with 100 μL of 1 μg/mL of Alexa Fluor 647-Labeled Human Her2, His Tag (Cat. No. HE2-HA2H5) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human Her2, His Tag (Cat. No. HE2-HA2H5) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa Fluor 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Receptor protein-tyrosine kinase erbB-2
- Category：
- Approved Drugs：
47 Details
- Drugs in Clinical Trials：
238 Details
- Highest Development Stage：
Approved
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