Product Details
Synonym
IL-12 R beta 2, IL12RB2, IL-12RB2, IL-12R-beta-2
Source
Human IL-12 R beta 2, His Tag (ILB-H52H6) is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Asn 622 (Accession # Q99665-1).
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.0 kDa. The protein migrates as 85-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human IL-12 R beta 2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human IL-12 R beta 2, His Tag (Cat. No. ILB-H52H6) is more than 85% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
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Bioactivity-ELISA

Immobilized Human IL-12 R beta 2, His Tag (Cat. No. ILB-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag (Cat. No. IL2-H8210) with a linear range of 0.01-0.3 μg/mL (QC tested).
Protocol
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