Human MANF Protein, His Tag, low endotoxin (MALS verified)

Synonym

Mesencephalic astrocyte-derived neurotrophic factor, Arginine-Rich Protein, arginine-rich, mutated in early stage tumors, ARMET, ARP, MANF, mesencephalic astrocyte-derived neurotrophic facto, Protein ARMET

Source

Human MANF Protein, His Tag (MAF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 182 (Accession # P55145).

Predicted N-terminus: Leu 25

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.0 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Sterility

Negative

Mycoplasma

Negative

Purity

>95% as determined by SDS-PAGE.

>98% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process