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Product Details
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Feline PD-1 Protein, His Tag (PD1-C52H6) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gly 168 (Accession # A9YUA6).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.0 kDa. The protein migrates as 27-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Feline PD-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Feline PD-1 Protein, His Tag (Cat. No. PD1-C52H6) is more than 95% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Feline PD-L1 Protein, Fc Tag (Cat. No. PD1-C5255) captured on Protein A Chip can bind Feline PD-1 Protein, His Tag (Cat. No. PD1-C52H6) with an affinity constant of 8.47 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed cell death protein 1
- Category：
- Approved Drugs：
22 Details
- Drugs in Clinical Trials：
193 Details
- Highest Development Stage：
Approved
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