Product Details
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Rat PD-1, Fc Tag (PD1-R5253) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_001100397.1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Rat PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Rat PD-1, Fc Tag (Cat. No. PD1-R5253) is more than 85% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Mouse PD-L2, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Rat PD-1, Fc Tag (Cat. No. PD1-R5253) with a linear range of 5-20 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Programmed cell death protein 1
- Category:
- Approved Drugs:
22 Details
- Drugs in Clinical Trials:
193 Details
- Highest Development Stage:
Approved
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