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Product Details
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Feline PD-L1 Protein, Fc Tag (PD1-C5255) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - His 239 (Accession # M3WAP8).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Feline PD-L1 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Feline PD-L1 Protein, Fc Tag (Cat. No. PD1-C5255) is more than 95% and the molecular weight of this protein is around 105-140 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Feline PD-1 Protein, His,Avitag (Cat. No. PD1-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Feline PD-L1 Protein, Fc Tag (Cat. No. PD1-C5255) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Feline PD-L1 Protein, Fc Tag (Cat. No. PD1-C5255) captured on Protein A Chip can bind Feline PD-1 Protein, His Tag (Cat. No. PD1-C52H6) with an affinity constant of 8.47 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
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