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Product Details
Synonym
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Source
Biotinylated Human PD-L1, Avitag,His Tag (PD1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human PD-L1, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat. No. PD1-H5255) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Human 4-1BB Protein, His Tag (Cat. No. 41B-H53H3) at 1 μg/mL, add increasing concentrations of Acasunlimab and then add Biotinylated Human PD-L1, Avitag, His Tag (Cat. No. PD1-H82E5) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.15 ng/mL (Routinely tested).
Validated with Bispecific Antibody.
Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 1 μg/mL, add increasing concentrations of Pumitamig and then add Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.31 ng/mL (Routinely tested).
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed death-ligand 1
- Category：
- Approved Drugs：
12 Details
- Drugs in Clinical Trials：
176 Details
- Highest Development Stage：
Approved
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