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Product Details
Synonym
RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF
Source
Biotinylated Mouse VEGF164, His,Avitag (VE4-M82Q3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # Q00731-2).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.5 kDa. The protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to different glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Mouse VEGF164, His,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized VEGFR1/R2 ,C-Fc at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His,Avitag (Cat. No. VE4-M82Q3) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Human VEGF R1, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His,Avitag (Cat. No. VE4-M82Q3) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Mouse VEGF164, His,Avitag (Cat. No. VE4-M82Q3) on SA Biosensor, can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE1-M5256) with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Mouse VEGF164, His,Avitag (Cat. No. VE4-M82Q3) on SA Biosensor, can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with an affinity constant of 1.33 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Vascular endothelial growth factor A
- Category：
- Approved Drugs：
54 Details
- Drugs in Clinical Trials：
61 Details
- Highest Development Stage：
Approved
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