产品详情
抗体来源(Source)
HRP conjugated Anti-His Antibody (AY63), mAb is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号(Clone)
AY63
亚型(Isotype)
Human IgG1 | Human Kappa
偶联(Conjugate)
HRP-Conjugated
抗体类型(Antibody Type)
Recombinant Monoclonal
种属反应性(Reactivity)
Tag
免疫原(Immunogen)
A protein peptide with his tag.
特异性(Specificity)
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
应用(Application)
ApplicationRecommended UsageWestern Blot0.2-2 ug/mLELISA1:3000-1:10000纯化(Purification)
Protein A purified / Protein G purified
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70 °C for 6 months after reconstitution.
- 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
活性(Bioactivity)-ELISA
Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).
Protocol
Western Blot验证
Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
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