resDetect™ HIV-1 p24 ELISA Kit (Residue Testing)

产品参数（Product Specifications）

产品概述（Product Overview）

resDetect™ HIV-1 p24 ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used for the quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processes where it is used as a lentivirus vector. It is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages.

应用说明（Application）

This kit is developed for quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processing where it is used as a lentivirus vector.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

组分（Materials Provided）

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of HIV-1 p24. The kit consists of Pre-coated Anti-HIV-1 p24 Antibody Microplate and HIV-1 p24 Standard and Biotin-Anti-HIV-1 p24 Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the HIV-1 p24 as standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-HIV-1 p24 Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程