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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteAAV3Format96TRegulatory StatusRUOSensitivity<2.62E+08 capsids/mLStandard Curve Range8.39E+09 capsids/mL-2.62E+08 capsids/mLAssay Time2hr 20 minSuitable Sample TypeFor the detection and quantitative determination of AAV3 capsid titration in AAV gene therapy product preparation processing.Sample volume100 uL产品概述（Product Overview）
AAV3 Titration ELISA Kit (Fast) is based on ELISA sandwich method and designed to detect and quantitative determine of AAV3 capsid titration in AAV gene therapy product preparation processing. It contains AAV3 empty capsids and a pair of antibodies against AAV3. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV3 capsid titration.
应用说明（Application）
AAV3 Titration ELISA Kit was developed for the detection and quantitative determination of AAV3 capsid titration in AAV gene therapy product preparation processing.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
组分（Materials Provided）
IDComponentsSizeAAV03H-C01Pre-coated Anti-AAV3 Antibody Microplate1 plateAAV03H-C02AAV3 Standard5.03E+10 capsidsAAV03H-C03HRP-Anti-AAV3 Antibody20 μgAAV03H-C0410×Washing Buffer50 mLAAV03H-C052×Dilution Buffer50 mLAAV03H-C06Substrate Solution12 mLAAV03H-C07Stop Solution7 mL原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of AAV3 capsid. The kit consists of Pre-coated Anti-AAV3 Antibody Microplate and AAV3 Standard,HRP-Anti-AAV3 Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the AAV3 capsid as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-AAV3 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of AAV3 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
回收率（Recovery）
Three AAV3 with different concentrations were tested to calculate the recovery rate.
专属性（Specificity）
The specific binding of AAV-A003H to AAV1/2/3/5/6/8/9/dj serotypes was detected. The results showed that AAV-A003H only binds specifically to AAV3.
The specific binding of AAV-A003H to native/denatured AAV3 was detected. The results showed that AAV-A003H only binds specifically to native AAV3. (Denatured AAV3: 95℃ for 10min)
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