resDetect™ Human 4-1BB Ligand ELISA Kit (Residue Testing)

产品参数（Product Specifications）

产品概述（Product Overview）

resDetect™ Human 4-1BB Ligand ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human 4-1BB Ligand residues in cell culture supernatants, serum, and plasma. The kit contains GMP human 4-1BB Ligand (ACROBiosystems, cat# GMP-41LH26) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human 4-1BB Ligand.

应用说明（Application）

The kit is developed for the detection and quantitative determination of 4-1BB Ligand in human serum and cell culture supernates.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

组分（Materials Provided）

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human 4-1BB Ligand. The kit consists of Pre-coated Anti-4-1BB Ligand Antibody Microplate and Human 4-1BB Ligand Standard and Biotin-Anti-4-1BB Ligand Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Human 4-1BB Ligand as standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-4-1BB Ligand Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程