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AAV9 Titration ELISA Kit (Fast)

COADSMS
DS
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AAV-A009H-96tests
¥6500.00
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产品详情

  • 产品参数（Product Specifications）

    Assay Type
    Sandwich-ELISA
    Analyte
    AAV9
    Format
    96T
    Regulatory Status
    RUO
    Sensitivity
    <3.91E+08 capsids/mL
    Standard Curve Range
    2.50E+10 capsids/mL-3.91E+08 capsids/mL
    Assay Time
    2hr 20 min
    Suitable Sample Type
    For the detection and quantitative detection and quantitative determination of AAV9 capsid titration in AAV gene therapy product preparation processing.
    Sample volume
    100 μL

  • 产品概述（Product Overview）

    AAV9 Titration ELISA Kit (Fast) is based on the sandwich-ELISA method and is used to detect and quantitatively determine the AAV9 capsid titration in AAV gene therapy product winthinn 2h 20min. The kit is designed to provide reliable solutions for the early development, production, and quality control of AAV gene therapy products.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储（Storage）

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分（Materials Provided）

    ID
    Components
    Size
    AAV09H-C01
    Pre-coated Anti-AAV9 Antibody Microplate
    1 plate
    AAV09H-C02
    AAV9 Standard
    3.80E+11 capsids
    AAV09H-C03
    HRP-Anti-AAV9 Antibody
    20 μg
    AAV09H-C04
    10×Washing Buffer
    50 mL
    AAV09H-C05
    2×Dilution Buffer
    50 mL
    AAV09H-C06
    Substrate Solution
    12 mL
    AAV09H-C07
    Stop Solution
    7 mL

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

    AAV9 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

验证

  • 稀释线性（Dilution Linearity）

    To assess the linearity of the assay, samples spiked with high concentrations of AAV9 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

    AAV9 DILUTION LINEARITY

  • 批内差异（Intra-Assay Statistics）

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

    AAV9 INTRA-ASSAY STATISTICS

  • 批间差异（Inter-Assay Statistics）

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

    AAV9 INTER-ASSAY STATISTICS

  • 回收率（Recovery）

    Three AAV9 with different concentrations were tested to calculate the recovery rate.

    AAV9 RECOVERY

  • 专属性（Specificity）

    The specific binding of AAV-A009H to AAV1/2/3/5/6/8/9/dj serotypes was detected. The results showed that AAV-A009H only binds specifically to AAV9.

    AAV9 SPECIFICITY

    The specific binding of AAV-A009H to native/denatured AAV9 was detected. The results showed that AAV-A009H only binds specifically to native AAV9. (Denatured AAV9: 95℃ for 5min)

    AAV9 SPECIFICITY

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背景介绍

The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.

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