resDetect™ Anti-CD28 Antibody ELISA Kit

产品参数（Product Specifications）

产品概述（Product Overview）

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

应用说明（Application）

The kit is developed for the detection of anti-CD28 antibody in Bioprocess manufacturing applications. It is used as a tool to aid in routine quality control of in-process streams as well as final product.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

组分（Materials Provided）

原理（Assay Principles）

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-CD28 antibodies. The kit consists of Pre-coated Human CD28 Microplate and Anti-CD28 Antibody Standard and HRP-Anti-Mouse IgG secondary antibody and related buffer.

Your experiment will include 5 simple steps:

a) All reagents were returned to room temperature(20℃-25℃) before use.

b) Add your sample to the plate, take the Anti-CD28 Antibody as standard sample. The samples and standard sample are diluted by Dilution Buffer.

c) Wash the plate and add a diluted HRP-Goat anti-Mouse IgG to the plate. The diluted by Dilution Buffer.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of Antibody bound.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程