Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit (For Vaccine Development)

组分（Materials Provided）

产品概述（Product Overview）

Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gI) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine.Therefore,It's helpful to develop the Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit to quantitative detection the VZV gE antigen in vaccine samples during the manufacture and quality control of vaccine development.

应用说明（Application）

This kit is developed for quantitative detection of Varicella Zoster Virus Glycoprotein E (VZV gE) in vaccine samples.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Glycoprotein E (VZV). The kit consists of Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate and Glycoprotein E (VZV) Standard,HRP-Anti-Glycoprotein E (VZV) Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the Glycoprotein E (VZV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the HRP-Anti-Glycoprotein E (VZV) Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程